ABSTRACT
Ipomoea batatas is a kind of both edible and medicinal plant, which provides a dietary source of vitamins, minerals, carbohydrates, proteins, anthocyanins, essential fatty acids, trace elements and other nutrients, and these active substances play a role in many pharmacological activities such as antitumor, immune regulation, hepatoprotective effect, hypoglycemic, hypolipidemic, anti-aging, intestinal regulation, anti-obesity, anti-radiation, anti-fatigue, etc, and promote health in many aspects. The Dictionary of Traditional Chinese Medicine and Chinese Materia Medica recorded that I. batatas have the characteristics of tonifying deficiency and replenishing qi, strengthening spleen and kidney. In recent years, it has become a research hotspot in multidisciplinary fields for its rich nutritional components and functional characteristics. In this paper, the research progress of biological activity of I. batatas in vivo was reviewed from aspects of basic and clinical researches, which may provide references for its further development, research and comprehensive utilization.
ABSTRACT
To separate and purify the crude polysaccharide from Aurantii Fructus to obtain CALB-1 and to study the structure of CALB-1 and immunoregulatory activity. A refined CALB-1 was obtained from F. aurantii by hot water extraction, then separated and purified by ion exchange resin and ion exchange agarose gel. The molecular weight of CALB-1 was measured by HPLC. The chemical structure and molecular morphology of CALB-1 were determined by IR, periodate oxidation, methylation analysis, scanning electron microscopy (SEM), and atomic force microscope (AFM). The immunoregulatory activity of CALB-1 was evaluated by splenocyte proliferation and mononuclear-macrophage phagocytic function in hypo-immunologic mice. CALB-1 was a homogeneous polysaccharide measured by HPLC and the molecular weight of CALB-1 was estimated to be 3.28 × 107. Chemical and spectroscopic analyses illustrated that CALB-1 was highly branched acidic polysaccharides, contained Ara, Man, and Gal as monosaccharide constitutes, and it consisted of backbone chain of 1→ and 1→4 linkages. Through SEM and AFM observations, we indicated that the molecular morphology of CALB-1 was amorphous solid. Besides, CALB-1 significantly stimulated the splenocyte proliferation in vitro and improved the K value of expurgation index and α value of phagocytic index in hypo-immunologic mice. CALB-1 is highly branched acidic polysaccharides and uniform relative molecular mass. Moreover, CALB-1 could present the certain immune regulation in vivo and in vitro. Our study provides a theoretical basis for the development and utilization of CALB-1.
ABSTRACT
Objective To study immunoregulatory activity of polysaccharide of mudan cortex (PSM) on immunodepressive mice. Method The immunodepressive mice model was established with cyclophosphamide treated and tumor-bearing. PSM was orally administered to mice for 10 d. MTT method was used to observe the prolifernation of lymphocytes. The function of macrophage in abdominal activity was measured. Sectrophotometric determination was adopted to evaluate the ability of plaque forming cell. Result PSM could enhance the function of macrophage, increase the content of plaque forming cell, and promote the prelifernation of lymphocytes. Conclusion PSM could enhance immunofunction of immunodepressive mice.
ABSTRACT
Objective:To study the immunoregulatory activity and mechanisms of Chinese medicinal herb Rhizoma Menipermi extracts.Compare the biological activity of Rhizoma Mednispermi extract with water (RMW),ethanol (RME) and Rhizoma Menispermi Polysaccharide (RMP).Methods:The chemical composition of Rhizoma Menipermi extract was separated with water,ethanol extraction and distillation methods.The effect of Rhizoma Menipermi extracts on lymphocytes and the effect on the metabolism and phagocytosis of mouse macrophages were studied by MTT colorimetric method and neutrol red method.Results:RMP can stimulate the proliferation of mouse spleen cells and human lymphocytes and enhance the metabolism and phagocytosis of mouse macrophages.RMW and RME have inhibitory effect on mouse spleen cells and human lymphocytes and mouse in vitro,and inhibited the metabolism and phagocytosis of mouse macrophages.Conclusion:Diffrent Rhizoma Menipermi composition showed diffrent effects on lymphocytes,RMW and RMP can be used for the supplementary treatment of cancer. [